GLDA, Active

Alternative Names: Not Available

Glycerol dehydrogenase (GldA) is an enzyme that facilitates the conversion of glycerol to dihydroxyacetone using NAD as a cofactor. This process enables microorganisms to utilize glycerol as a carbon source in anaerobic conditions. In E.coli, GldA plays a crucial role in regulating the levels of dihydroxyacetone by converting it back into glycerol. GldA has a broad substrate specificity, being able to oxidize 1,2-propanediol and reduce glycolaldehyde, methylglyoxal, and hydroxyacetone into ethylene glycol, lactaldehyde, and 1,2-propanediol, respectively.

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  • Sku: PSB-enz-904-2µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant E.coli Glycerol dehydrogenase, Active is an enzyme belonging to the dehydrogenase family, specifically involved in the oxidation of glycerol to dihydroxyacetone. This enzyme has a molecular weight of approximately 40 kDa and plays a significant role in glycerol metabolism within E.coli cells. Its active form is commonly used in biotechnology applications for the production of biofuels and other valuable compounds.

Current Lead Time

7-14 Days

Product Specifications

Species
 Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity: > 14 Units/ml. One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 41.1 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GLDA protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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GLDA, Active
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