GLDA

Alternative Names: Not Available

GldA is an enzyme that enables microorganisms to utilize glycerol as a carbon source under anaerobic conditions by catalyzing its oxidation to dihydroxyacetone. In E.coli, GldA plays a crucial role in regulating the intracellular levels of dihydroxyacetone by catalyzing the reverse reaction, converting dihydroxyacetone to glycerol. This enzyme exhibits broad substrate specificity, able to oxidize 1,2-propanediol and reduce glycolaldehyde, methylglyoxal, and hydroxyacetone to ethylene glycol, lactaldehyde, and 1,2-propanediol, respectively.

Skip to product information
  • Sku: PSB-enz-827-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
 per 
Shipping calculated at checkout.

Contact us directly for international purchases.

Title

Product Details

Product Description

Recombinant E.coli Glycerol dehydrogenase is an enzyme that plays a key role in glycerol metabolism. Belonging to the alcohol dehydrogenase family, this enzyme is responsible for catalyzing the conversion of glycerol to dihydroxyacetone. With a molecular weight of approximately 40 kDa, Recombinant E.coli Glycerol dehydrogenase is widely used in biotechnological applications for its ability to efficiently convert glycerol into valuable products.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
41.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GLDA protein solution (1mg/ml)containing Phosphate buffered saline (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

View full details

GLDA

SUBHEADING

Recently viewed products