GCAT

Alternative Names: Aminoacetone synthase, EC 2.3.1.29, 2-amino-3-ketobutyrate coenzyme A ligase mitochondrial, AKB ligase, Glycine acetyltransferase, GCAT, KBL.

L-threonine degradation involves two enzymes: L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. The first enzyme converts L-threonine into 2-amino-3-ketobutyrate, while the second enzyme, glycine C-Acetyltransferase (GCAT), catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to produce glycine and acetyl-CoA. GCAT is a class II pyridoxal-phosphate-dependent aminotransferase and is expressed in the heart, brain, liver, pancreas, and lung.

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  • Sku: PSB-enz-705-5µg
  • Vendor: ProSpec-Tany
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UniProt Listing
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Product Details

Product Description

Recombinant Human Glycine C-Acetyltransferase is an enzyme that belongs to the transferase family. It plays a role in the biosynthesis of glycine, an important amino acid involved in various metabolic processes. This enzyme has a molecular weight of approximately 50 kDa. Recombinant Human Glycine C-Acetyltransferase is a key player in the regulation of glycine levels within the body, making it a valuable target for research in the field of biochemistry and molecular biology.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GCAT protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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GCAT
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