GALNT1

Alternative Names:

Polypeptide N-acetylgalactosaminyltransferase 1, GALNT1, GALNAC-T1

Polypeptide N-acetylgalactosaminyltransferase 1, GALNT1, GALNAC-T1

Galnt1 is a member of the GalNAc-T family of enzymes and catalyzes the transfer of N-acetyl-D-galactosamine to serine or threonine residues on protein receptors, initiating O-linked oligosaccharide biosynthesis. This enzyme is also involved in the glycosylation of proteins crucial for bone formation, such as osteopontin and bone sialoprotein.

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  • Sku: PSB-enz-1098-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Polypeptide N-Acetylgalactosaminyltransferase 1 is a glycosyltransferase enzyme that belongs to the glycosyltransferase 27 family. It plays a key role in the biosynthesis of mucin-type O-glycans by catalyzing the transfer of N-acetylgalactosamine to serine or threonine residues. With a molecular weight of approximately 50 kDa, this enzyme is involved in post-translational modifications of proteins and is essential for proper cell function.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Insect cells
Purity 90.0 % by SDS-PAGE
Activity
The specific activity which is defined as the amount of enzyme that transfer 1.0 pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute at pH 8.0 at 37C is > 300 pmol/min/ug.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
60.4 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GALNT1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADPGLPAGDV LEPVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRSLPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDASERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSKGQVITF LDAHCECTVGWLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDRRKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIVTCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDISSRVGLRHKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIFNCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQHVNSNQCLDKA TEEDSQVPSI RDCNGSRSQQ WLLRNVTLPE IFHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GALNT1

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