G6PD

Alternative Names: G6PD, Glucose-6-phosphate 1-dehydrogenase, G6PD1

G6PD is a key enzyme in the pentose phosphate pathway which generates NADPH, an essential molecule for maintaining cellular redox balance. Deficiency in G6PD can lead to conditions such as hemolytic anemia, neonatal jaundice, and acute hemolysis. This cytosolic enzyme is encoded by an X-linked gene and plays a crucial role in producing NADPH, which is necessary for protection against oxidative damage and facilitating reductive biosynthetic reactions. Additionally, G6PD is involved in the production of pentose sugars for nucleic acid synthesis.

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  • Sku: PSB-enz-399-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant E. coli Glucose-6-Phosphate Dehydrogenase is an enzyme that plays a key role in the pentose phosphate pathway, catalyzing the conversion of glucose-6-phosphate to 6-phosphogluconolactone. Belonging to the dehydrogenase family, this enzyme is crucial for the production of NADPH, an essential cofactor in various metabolic processes. With a molecular weight of approximately 60 kDa, Recombinant E. coli Glucose-6-Phosphate Dehydrogenase is widely used in biochemical research and biotechnology applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Specific activity > 70 units/mg obtained by measuring  the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction beta- NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
55.7 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The G6PD protein contains 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVDSITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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G6PD

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