Fumarase

Alternative Names: Fumarase, Fumarate hydratase, FH, HLRCC, LRCC, MCUL1, MCL

Fumarase is a key enzyme in the Krebs cycle, responsible for converting fumarate to L-malate. It can exist in two forms: cytosolic and N-terminal extended, with the latter targeting the mitochondrion. Fumarase functions as a homotetramer and is similar to other Class-2 fumarases. Mutations in the Fumarase gene can lead to fumarase deficiency, resulting in symptoms such as progressive encephalopathy and cerebral atrophy. Fumarase is also believed to have tumor suppressor properties, with Fumarase mutations being linked to Leydig cell tumors, marking a significant finding in adult testicular tumors.

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  • Sku: PSB-enz-395-10µg
  • Vendor: ProSpec-Tany
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UniProt Listing
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Product Details

Product Description

Recombinant Human Fumarate Hydratase is a protein belonging to the lyase family, specifically involved in the tricarboxylic acid cycle. With a molecular weight of approximately 60 kDa, this enzyme plays a crucial role in the conversion of fumarate to malate, a key step in cellular energy production. Recombinant Human Fumarate Hydratase is commonly used in research and biotechnological applications for its enzymatic activity and biochemical properties.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37°C. Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homotetramer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The Fumarase protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MASQNSFRIE YDTFGELKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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Fumarase
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