FGF1, Sf9

Alternative Names: ECGF, ECGFA, ECGF-beta, FGF1, FGF-a, FGFIBP, FIBP, FIBP-1, HBGF-1, GLIO703

Acidic fibroblast growth factor is a member of the fibroblast growth factor family with mitogenic and cell survival activities. It plays a role in various biological processes, including embryonic development, tissue repair, tumor growth, and invasion. It is a modifier of endothelial cell migration and proliferation and functions as an angiogenic factor. In vitro studies suggest it may be involved in organogenesis. Three different isoforms of this protein have been identified through alternative splicing. Heparin-binding growth factors, like acidic fibroblast growth factor, are angiogenic agents in vivo and potent mitogens for various cell types in vitro. Tissue distribution and concentration of these growth factors vary.

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  • Sku: PSB-cyt-364-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Fibroblast Growth Factor-acidic, Sf9 is a member of the fibroblast growth factor family, a group of signaling proteins involved in cell growth, development, and wound healing. This particular protein is produced in Sf9 insect cells using recombinant DNA technology. It has a molecular weight of approximately 17 kDa and is commonly used in research and biotechnology applications for its ability to stimulate cell proliferation and differentiation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Baculovirus
Purity 95% by SDS-PAGE
Activity
 The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng/ml, corresponding to a specific activity of 100,000IU/mg. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng/ml, corresponding to a specific activity of 100,000IU/mg.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The sterile protein solution (1.8mg/ml) contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

The sequence of the first five N-terminal amino acids as determined and was found to be Met-Phe-Asn-Leu-Pro.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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FGF1, Sf9
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