FEN1

Alternative Names: Flap endonuclease 1, Flap structure-specific endonuclease 1, FEN-1, FEN1, hFEN-1, Maturation Factor-1, Maturation factor 1, MF1, MF-1, RAD2, DNase IV

FEN1 is a key enzyme involved in DNA repair and DNA synthesis. It plays a role in removing overhanging flaps and processing Okazaki fragments. FEN1 works in coordination with other proteins like AP endonuclease 1 during base excision repair, ensuring efficient substrate transfer. This protein is essential for DNA replication and is part of the XPG/RAD2 endonuclease family. However, DNA secondary structure can hinder its function, particularly at trinucleotide repeats, leading to site-specific expansions.

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  • Sku: PSB-enz-468-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Flap Structure-Specific Endonuclease 1 is a member of the endonuclease family, specifically targeting flap structures in DNA molecules. This enzyme plays a critical role in DNA repair mechanisms by cleaving the single-stranded DNA regions that form during replication or recombination. With a molecular weight of approximately 37 kDa, Recombinant Human Flap Structure-Specific Endonuclease 1 is a key player in maintaining genomic stability and integrity.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The protein contains 20mM Tris-HCl buffer pH-8.0, 1mM DTT, 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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FEN1
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