FBL

Alternative Names: rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, FBL, FIB, FIB1, FLRN, fibrillarin, RNU3IP1.

FBL is a small nucleolar protein in eukaryotes that plays a crucial role in pre-rRNA processing for ribosomal biogenesis. It is a component of various ribonucleoproteins, including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Fibrillarin contains glycine and arginine-rich N-terminal repetitive domains, similar to those in other species, along with an RNA-binding domain in the central region that includes an RNP consensus sequence. FBL is associated with the U3, U8, and U13 small nuclear RNAs in the dense fibrillar component (DFC) of the nucleolus. Approximately 8% of patients with scleroderma have antibodies that recognize fibrillarin.

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  • Sku: PSB-enz-566-5µg
  • Vendor: ProSpec-Tany
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UniProt Listing
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Product Details

Product Description

Recombinant human Fibrillarin is a protein that belongs to the family of nucleolar proteins. It plays a key role in ribosome biogenesis and is involved in the processing and modification of ribosomal RNA. With a molecular weight of approximately 34 kDa, Fibrillarin is essential for the proper functioning of the nucleolus and is highly conserved across species. This recombinant protein is commonly used in research studies to investigate its role in various cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90.0 % by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 28.9 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The FBL solution contains Phosphate buffered saline (pH 7.4), 30% glycerol, and 1mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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FBL
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