FAS

Alternative Names: Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6.

Fas and Fas Ligand (FasL) are proteins belonging to the TNF superfamily that initiate apoptosis in cells expressing Fas. FasL binds to Fas, triggering a cascade of events that leads to cell death. This pathway involves the recruitment of pro-caspase 8 by an adaptor protein called FADD, followed by its activation which then cleaves various cellular components. The soluble form of Fas (sFasR) can inhibit FasL-induced apoptosis by acting as a decoy receptor. The full length Fas Receptor is a 319 amino acid protein with distinct extracellular, transmembrane, and cytoplasmic domains. The mature form of human Fas shares sequence identities of 55% and 58% with its mouse and rat counterparts, respectively.

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  • Sku: PSB-cyt-125-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human sFas Receptor is a soluble form of the Fas receptor, a member of the tumor necrosis factor (TNF) receptor superfamily. This protein plays a key role in the regulation of apoptosis, or programmed cell death, by binding to Fas ligand and initiating the caspase cascade. With a molecular weight of approximately 45 kDa, Recombinant Human sFas Receptor is commonly used in research and therapeutic applications to study and manipulate cell death pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 17.6 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Lyophilized
Formulation FAS protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.

Storage Instruction

Lyophilized FAS although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FAS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized FAS in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available
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FAS
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