F3

Alternative Names: Tissue factor, Coagulation factor III, Thromboplastin, CD142, TF, F3, TFA.

Tissue factor is a well-known initiator of blood coagulation. It encodes coagulation factor III, which is a glycoprotein that allows cells to start blood clotting. Tissue factor interacts with coagulation factor VII to activate the coagulation cascade, resulting in fibrin deposition and platelet activation. This process is crucial for normal blood clotting. Tissue factor can also be induced by certain inflammatory mediators and endotoxin, leading to its appearance on immune cells and vascular endothelial cells. Tissue factor deficiency has not been reported, as it is necessary for the viability and growth of endothelial cells and some tumor cells. However, abnormal expression of tissue factor can contribute to dangerous blood clotting in diseases like sepsis, atherosclerosis, and cancer. In pancreatic cancer, alternative splicing of tissue factor promotes tumor growth, cell proliferation, and angiogenesis.

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  • Sku: PSB-pro-312-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Coagulation Factor III, also known as tissue factor (TF), is a glycoprotein that plays a crucial role in the initiation of blood coagulation. Belonging to the serine protease family, this protein acts as a cofactor for the activation of coagulation factors VII and VIIa. With a molecular weight of approximately 47 kDa, Recombinant Human Coagulation Factor III is involved in the extrinsic pathway of blood coagulation, facilitating the formation of the tenase complex and subsequent thrombin generation. Its significance lies in its contribution to hemostasis and the maintenance of vascular integrity.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Tissue factor protein is supplied in 20mM Tris-HCl pH8.0, 1mM EDTA and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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F3

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