ERO1L

Alternative Names: EC 1.8.4, EC 1.8.4.-, ERO1 (S. Cerevisiae)-Like, ERO1-Like (S. Cerevisiae), ERO1-Like Protein, ERO1-Like Protein Alpha, ERO1-L-Alpha, ERO1-L, ERO1A, ERO1LA, ERO1-Alpha, ERO1L

ERO1-like protein alpha (ERO1L) is a crucial oxidoreductase involved in protein oxidation and necessary for immunoglobulin folding. It binds covalently with protein disulfide-isomerase (PDI) to produce disulfide bonds between proteins in the endoplasmic reticulum. Hypoxia stimulates ERO1L, suggesting regulation through the hypoxia inducible transcription factor (HIF) pathway. ERO1L is widely expressed at low levels but primarily expressed in the upper digestive tract and esophagus at high levels. Additionally, ERO1L aids in releasing the unfolded cholera toxin during V.cholerae infection by interacting with reduced P4HB/PDI, contributing to toxin retrotranslocation. Furthermore, ERO1L is crucial in ER stress-induced, CHOP-dependent apoptosis by activating inositol 1,4,5-trisphosphate receptor IP3R1.

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  • Sku: PSB-pro-1957-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human ERO1-Like, a member of the endoplasmic reticulum oxidoreductase 1-like family, is a protein with a molecular weight of approximately X kDa. This protein plays a significant role in the oxidative folding of secretory proteins in the endoplasmic reticulum, aiding in the formation of disulfide bonds. Through its enzymatic activity, Recombinant Human ERO1-Like contributes to the maintenance of protein homeostasis, ensuring proper protein folding and quality control. Its involvement in redox regulation makes it a crucial component in cellular processes related to protein synthesis and secretion.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ERO1L solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSEEQPPET AAQRCFCQVS GYLDDCTCDV ETIDRFNNYR LFPRLQKLLE SDYFRYYKVN LKRPCPFWND ISQCGRRDCA VKPCQSDEVP DGIKSASYKY SEEANNLIEE CEQAERLGAV DESLSEETQK AVLQWTKHDD SSDNFCEADD IQSPEAEYVD LLLNPERYTG YKGPDAWKIW NVIYEENCFK PQTIKRPLNP LASGQGTSEE NTFYSWLEGL CVEKRAFYRL ISGLHASINV HLSARYLLQE TWLEKKWGHN ITEFQQRFDG ILTEGEGPRR LKNLYFLYLI ELRALSKVLP FFERPDFQLF TGNKIQDEEN KMLLLEILHE IKSFPLHFDE NSFFAGDKKE AHKLKEDFRL HFRNISRIMD CVGCFKCRLW GKLQTQGLGT ALKILFSEKL IANMPESGPS YEFHLTRQEI VSLFNAFGRI STSVKELENF RNLLQNIH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ERO1L

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