ERCC1

Alternative Names: Excision Repair Cross-complementing rodent repair deficiency complementation group 1 (includes overlapping antisense sequence), DNA excision repair protein ERCC-1, RAD10, COFS4, UV20.

ERCC1 polypeptide is a crucial component of nucleotide excision repair (NER) in damaged DNA. It shares similarities with Saccharomyces cerevisiae RAD10, a protein involved in repair and recombination during mitosis. NER involves two nucleases catalyzing dual incisions on either side of the damage. In mammalian cells, XPG cleaves the DNA lesion at the 3' end, while the ERCC1-XPF complex performs the 5' incision.

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  • Sku: PSB-pro-1106-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Excision Repair Cross-Complementing 1 (ERCC1) is a protein involved in the nucleotide excision repair pathway, responsible for repairing DNA damage caused by ultraviolet radiation and certain chemicals. Belonging to the ERCC family, this protein plays a crucial role in maintaining genomic stability and preventing the accumulation of mutations. With a molecular weight of approximately 33 kDa, ERCC1 interacts with other proteins to form a complex that recognizes and removes damaged DNA segments, ensuring the integrity of the genetic material.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
32.5 kDa
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ERCC1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ERCC1

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