Enterokinase

Alternative Names: Enteropeptidase, Enterokinase, Transmembrane Protease Serine 15, Serine protease 7, EC 3.4.21.9, ENTK, TMPRSS15, MGC133046

Enteropeptidase, an enzyme crucial in human digestion, is produced by cells in the duodenum wall and secreted from glands in the duodenum whenever food enters from the stomach. It plays a key role in activating trypsinogen to trypsin, indirectly activating other pancreatic digestive enzymes. Classified as a serine protease enzyme of the Chymotrypsin clan, enteropeptidase shares structural similarities with other proteins in this group.

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  • Sku: PSB-enz-260-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Enteropeptidase/Enterokinase is a serine protease enzyme belonging to the family of trypsin-like proteases. With a molecular weight of approximately 64 kDa, this enzyme plays a key role in the activation of trypsinogen to trypsin in the small intestine, thereby initiating the digestive process. Its precise cleavage site specificity and high specificity make it a valuable tool in various research and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
26.4 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Enterokinase 1mg/ml is supplied in 20mM Tris-HCl, pH 8.0, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAIVGGSNAK EGAWPWVVGL YYGGRLLCGA SLVSSDWLVS AAHCVYGRNL EPSKWTAILG LHMKSNLTSP QTVPRLIDEI VINPHYNRRR KDNDIAMMHL EFKVNYTDYI QPICLPEENQ VFPPGRNCSI AGWGTVVYQG TTANILQEAD VPLLSNERCQ QQMPEYNITE NMICAGYEEG GIDSCQGDSG GPLMCQENNR WFLAGVTSFG YKCALPNRPG VYARVSRFTE WIQSFLH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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Enterokinase

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