ENPP2

Alternative Names: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP2, E-NPP 2, AUTOTAXIN, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA.

ENPP2, a member of the ectonucleotide pyrophosphatase family, is an enzyme that cleaves the phosphodiester bond in triphosphate nucleotides to produce pyrophosphate. Unlike other members of the ENPP family, ENPP2 has weak activity against nucleotides but shows lysophospholipase D activity, forming LPA and choline from lysophosphatidylcholine. ENPP2 and LPA play a role in inflammatory-driven diseases like arthritis and asthma.

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  • Sku: PSB-enz-1173-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Ectonucleotide Pyrophosphatase-2 is a member of the ectonucleotide pyrophosphatase/phosphodiesterase (ENPP) family, which plays a role in purinergic signaling and bone mineralization. This enzyme is involved in the hydrolysis of nucleoside triphosphates and diphosphates, releasing inorganic pyrophosphate. Recombinant Human Ectonucleotide Pyrophosphatase-2 has a molecular weight of approximately 150 kDa and is commonly used in research settings to study its physiological functions and potential therapeutic applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System HEK 293
Purity 90.0 by SDS-PAGE
Activity
Specific activity is greater than 15,000 units/mg, and defined as the amount of enzyme that hydrolyze 1nmole of bis (pNitrophenyl) phosphate per minute at pH8.7 at 37℃.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
94.9 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ENPP2 solution (0.25mg/ml) contains PBS (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

DGSMDSPWTN ISGSCKGRCF ELQEAGPPDC RCDNLCKSYT SCCHDFDELC LKTARGWECT KDRCGEVRNE ENACHCSEDC LARGDCCTNY QVVCKGESHW VDDDCEEIKA AECPAGFVRP PLIIFSVDGF RASYMKKGSK VMPNIEKLRS CGTHSPYMRP VYPTKTFPNL YTLATGLYPE SHGIVGNSMY DPVFDATFHL RGREKFNHRW WGGQPLWITA TKQGVKAGTF FWSVVIPHER RILTILQWLT LPDHERPSVY AFYSEQPDFS GHKYGPFGPE MTNPLREIDK IVGQLMDGLK QLKLHRCVNV IFVGDHGMED VTCDRTEFLS NYLTNVDDIT LVPGTLGRIR SKFSNNAKYD PKAIIANLTC KKPDQHFKPY LKQHLPKRLH YANNRRIEDI HLLVERRWHV ARKPLDVYKK PSGKCFFQGD HGFDNKVNSM QTVFVGYGST FKYKTKVPPF ENIELYNVMC DLLGLKPAPN NGTHGSLNHL LRTNTFRPTM PEEVTRPNYP GIMYLQSDFD LGCTCDDKVE PKNKLDELNK RLHTKGSTEE RHLLYGRPAV LYRTRYDILY HTDFESGYSE IFLMPLWTSY TVSKQAEVSS VPDHLTSCVR PDVRVSPSFS QNCLAYKNDK QMSYGFLFPP YLSSSPEAKY DAFLVTNMVP MYPAFKRVWN YFQRVLVKKY ASERNGVNVI SGPIFDYDYD GLHDTEDKIK QYVEGSSIPV PTHYYSIITS CLDFTQPADK CDGPLSVSSF ILPHRPDNEE SCNSSEDESK WVEELMKMHT ARVRDIEHLT SLDFFRKTSR SYPEILTLKT YLHTYESEIH HHHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ENPP2

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