ENO1

Alternative Names: NNE, Non-neural enolase, ENO1, Enolase 1, ENO1L1, Alpha-Enolase, Enolase-Alpha, 2-phospho-D-glycerate hydro-lyase, PPH, Phosphopyruvate hydratase, MPB-1, MBP-1, C-myc promoter-binding protein, MPB1, Plasminogen-binding protein, MBPB1.

ENO1 is a soluble homodimeric protein that encodes the monomeric tau-crystallin lens protein. It is a glycolytic enzyme expressed in various tissues, mainly found in the cytoplasm as a full-length protein. Another translation start within the nucleus produces a shorter protein that binds to a component in the c-myc promoter. ENO1 plays a role in anaerobic metabolism under hypoxic conditions and acts as a cell surface plasminogen receptor during tissue invasion. Abnormal ENO1 expression is associated with tumor progression in breast and lung cancer cases, as well as autoantibodies in Hashimoto's encephalopathy and severe asthma. Furthermore, ENO1 is the target protein of serum anti-endothelial antibodies in Behcet's disease.

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  • Sku: PSB-enz-452-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Enolase-1 is a member of the enolase family of enzymes, which play a key role in glycolysis and various cellular processes. With a molecular weight of approximately 47 kDa, this enzyme catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, an important step in the glycolytic pathway. Recombinant Human Enolase-1 is commonly used in research and biotechnology applications due to its stability and specificity.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The ENO1 protein solution (1mg/ml) is formulated in 20mM Tris-HCl pH-7.5 1mM MgSO4 and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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ENO1
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