ELOB

Alternative Names: ELOB, SIII, Elongin B, Transcription elongation factor B polypeptide 2, RNA polymerase II transcription factor SIII subunit B, SIII p18, Elongin 18 kDa subunit, TCEB2.

TCEB2 is a component of the SIIII complex, which is composed of a transcriptionally active subunit (A) and two regulatory subunits (B and C). Its role is to facilitate RNA polymerase II elongation by preventing temporary pauses at various locations within transcription units. The presence of the von Hippel-Lindau tumor suppressor protein interferes with this elongation process by binding to elongins B and C.

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  • Sku: PSB-pro-664-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Elongin-B is a protein that belongs to the Elongin family, which plays a crucial role in the regulation of transcription and protein degradation processes. With a molecular weight of approximately XX kDa, this protein acts as a subunit of the Elongin complex, along with Elongin-C and Elongin-A, to form a functional E3 ubiquitin ligase. Recombinant Human Elongin-B is widely utilized in various research applications, such as studying protein-protein interactions and investigating the mechanisms of ubiquitin-mediated protein degradation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Heterotrimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The TCEB2 protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1mM PMSF, and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCIEPFSSPPELP DVMKPQDSGS SANEQAVQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ELOB

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