EIF5A

Alternative Names: EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor.

EIF5A is a critical protein involved in peptide bond formation during translation. It is formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. While initially believed to only participate in the first step of peptide bond formation, further experiments indicate that EIF5A is a universally conserved translation elongation factor. Its regulation is associated with proliferation and cancer, as demonstrated by its upregulation in the PBMCs of HIV-1 patients. EIF5A also plays a role in important cellular processes such as cell viability and senescence by influencing the stability of certain mRNAs. In particular, the loss of EIF5A under heat stress conditions in a pancreatic cancer cell line affects the fate of the cells. Thus, EIF5A stability is crucial in determining the outcome of a specific cell type after exposure to severe heat stress.

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  • Sku: PSB-pro-674-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 5A (eIF5A) is a protein that plays a critical role in the initiation of protein synthesis in eukaryotic cells. Belonging to the eIF5A family, this protein is involved in the transfer of the initiator methionyl-tRNA to the ribosome during translation initiation. With a molecular weight of approximately 17 kDa, eIF5A is known for its unique post-translational modification, which involves the addition of the amino acid hypusine. This modification is crucial for the proper functioning of eIF5A and its involvement in various cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The EIF5A protein solution (1mg/ml) contains 50mM Tris-HCl, pH-7.5 and 10% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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EIF5A
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