EIF4A3

Alternative Names: Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Nuclear matrix protein 265, NMP 265, hNMP 265, EIF4A3, DDX48, KIAA0111, NUK34, NMP265, eIF4AIII.

Eukaryotic initiation factor 4A-III (EIF4A3) belongs to the DEAD box helicase family and eIF4A subfamily. As part of this family, EIF4A3 is believed to function as an RNA helicase, involved in various cellular processes. These processes include modifying RNA secondary structure during translation initiation, as well as participating in nuclear and mitochondrial splicing, and the assembly of ribosomes and spliceosomes. EIF4A3 is specifically a component of the exon junction complex (EJC) that accumulates at splice junctions on mRNA molecules. Some members of the DEAD box helicase family are associated with embryogenesis, spermatogenesis, and cellular growth and division.

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  • Sku: PSB-pro-1007-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 4A3 (eIF4A3) is a protein involved in the initiation of protein synthesis in eukaryotic cells. Belonging to the DEAD-box helicase family, eIF4A3 plays a crucial role in unwinding RNA secondary structures during translation initiation. With a molecular weight of approximately 46 kDa, this protein facilitates the binding of the mRNA cap structure to the ribosome, enabling efficient protein synthesis. Its functional significance in translation initiation makes eIF4A3 a subject of extensive research in understanding cellular processes and potential therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 49.4 kDa
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation EIF4A3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 30% glycerol and 200mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMATTAT MATSGSARKR LLKEEDMTKV EFETSEEVDV TPTFDTMGLR EDLLRGIYAY GFEKPSAIQQ RAIKQIIKGR DVIAQSQSGT GKTATFSISV LQCLDIQVRE TQALILAPTR ELAVQIQKGL LALGDYMNVQ CHACIGGTNV GEDIRKLDYG QHVVAGTPGR VFDMIRRRSL RTRAIKMLVL DEADEMLNKG FKEQIYDVYR YLPPATQVVL ISATLPHEIL EMTNKFMTDP IRILVKRDEL TLEGIKQFFV AVEREEWKFD TLCDLYDTLT ITQAVIFCNT KRKVDWLTEK MREANFTVSS MHGDMPQKER ESIMKEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQIDEMPMN VADLI.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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EIF4A3
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