EIF3J

Alternative Names: Eukaryotic translation initiation factor 3 subunit 1, Eukaryotic translation initiation factor 3 subunit J, eIF-3-alpha, eIF3 p35, EIF3J, EIF3S1, eIF3-alpha.

Eukaryotic translation initiation factor 3 subunit J (EIF3J) is a crucial member of the EIF-3 subunit J family. It plays a crucial role in the binding of methionyl-tRNA and mRNA to the 40S ribosomal subunit to create the 40S initiation complex. EIF3J directly binds to the aminoacyl (A) site and mRNA access channel of the 40S subunit, placing it in the ribosomal decoding center. Additionally, EIF3J interacts with eIF1A and decreases the affinity of the 40S subunit for mRNA. However, this affinity is restored when the initiator tRNA is recruited, even though EIF3J remains in the mRNA-binding cleft when tRNA is present.

  •
  • Sku: PSB-pro-970-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 3J (eIF3J) is a protein belonging to the eukaryotic translation initiation factor 3 (eIF3) family. With a molecular weight of approximately X kDa, eIF3J plays a significant role in the initiation of protein synthesis in eukaryotic cells. This protein is involved in the assembly of the eIF3 complex, which is crucial for the recognition of the mRNA start codon and subsequent recruitment of ribosomes. By facilitating the accurate translation initiation process, eIF3J contributes to the overall efficiency and fidelity of protein synthesis in eukaryotes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The EIF3J solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 200mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

EIF3J
SUBHEADING

Recently viewed products