EIF3I, Sf9

Alternative Names: eIF3-beta, eIF3-p36, EIF3S2, PRO2242, TRIP-1, TRIP1, Eukaryotic translation initiation factor 3 subunit I, eIF3i, TGF-beta receptor-interacting protein 1, eIF-3-beta.

Eukaryotic translation initiation factor 3 subunit I (EIF3I) is a vital component of the eukaryotic translation initiation factor 3 (eIF-3) complex. This complex plays a crucial role in multiple stages of protein synthesis initiation. It connects with the 40S ribosome to facilitate the assembly of the 43S pre-initiation complex (43S PIC), involving the recruitment of eIF-1, eIF-1A, eIF-2: GTP: methionyl-tRNAi, and eIF-5. The eIF-3 complex enhances mRNA recruitment and scanning for AUG recognition in the 43S PIC. Additionally, it is responsible for disassembling and recycling post-termination ribosomal complexes, ensuring that the 40S and 60S ribosomal subunits do not prematurely join before the initiation process. EIF3I has been associated with diseases such as clonorchiasis and tonsillitis.

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  • Sku: PSB-pro-2611-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 3I, Sf9 is a protein that plays a significant role in the initiation of protein synthesis in eukaryotic cells. Belonging to the family of translation initiation factors, this protein is involved in the assembly of ribosomes and the recruitment of mRNA to the ribosome for translation. With a molecular weight of approximately X kDa, Recombinant Human Eukaryotic Translation Initiation Factor 3I, Sf9 is a crucial component in the intricate process of protein synthesis.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Baculovirus cells
Purity 90.0 % by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 37.3 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation EIF3I protein solution (0.25mg/ml) 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 40% Glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDADWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG FDFGGNIIMF STDKQMGYQCFVSFFDLRDP SQIDNNEPYM KIPCNDSKIT SAVWGPLGEC IIAGHESGEL NQYSAKSGEVLVNVKEHSRQ INDIQLSRDM TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPNYDHVVLGGGQ EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY SSGGEDGYVR IHYFDPQYFE FEFEAHHHHH H.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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EIF3I, Sf9
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