EIF2S1

Alternative Names: Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, EIF-2-alpha, EIF-2alpha, EIF-2A, EIF2, EIF-2, EIF2A, EIF-2A.

EIF2S1 plays a role in the beginning stages of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit and is followed by mRNA binding to create a 43S pre-initiation complex. The 80S initiation complex is formed when the 60S ribosomal subunit joins. Before this happens, the GTP bound to eIF-2 is hydrolyzed and an eIF-2-GDP binary complex is released. In order for eIF-2 to be reused and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP through a reaction catalyzed by eIF-2B.

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  • Sku: PSB-pro-845-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 2 Subunit 1 Alpha, also known as eIF2S1, is a protein that plays a pivotal role in the initiation of protein synthesis in eukaryotic cells. Belonging to the family of translation initiation factors, eIF2S1 is responsible for binding to the initiator methionyl-tRNA and facilitating its recognition by the ribosome during the translation process. With a molecular weight of approximately 36 kDa, this protein is crucial for the accurate and efficient initiation of protein synthesis, ensuring the proper functioning of cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation 0.5mg/ml solution containing 20mM Tris-HCl pH-8, 0.1M NaCl & 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV DGDDDAEEME AKAED

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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EIF2S1

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