EIF1AX

Alternative Names: BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, Biglycan Proteoglycan, MRLS

Biglycan (BGN) is a small proteoglycan involved in collagen fibril assembly and muscle regeneration. It shares structural similarities with decorin and fibromodulin. BGN interacts with various proteins related to muscular dystrophy, such as alpha-dystroglycan, alpha- and gamma-sarcoglycan, and collagen VI. Additionally, BGN plays a critical role in the assembly of the dystrophin-associated protein complex.

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  • Sku: PSB-pro-253-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Eukaryotic Translation Initiation Factor 1A X-linked (eIF1AX) is a protein belonging to the initiation factor family, which plays a crucial role in the initiation of protein synthesis in eukaryotic cells. With a molecular weight of approximately 12 kilodaltons, eIF1AX is involved in the recognition of the start codon on mRNA and the assembly of the ribosomal complex during translation initiation. This protein is of significant interest in molecular biology and biotechnology research due to its involvement in fundamental cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Insect cells
Purity 90.0 % by SDS-PAGE
Activity
The ED50 for this effect is ≤ 20 ug/ml. The specific activity is measured by inhibiting the cell growth using 3T3‑ L1 mouse embryonic  fibroblast  adipose-like cells.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
38.3 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The BGN solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADPDEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKKHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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EIF1AX

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