EGLN3

Alternative Names: Egl nine homolog 3 (C. elegans), Prolyl hydroxylase domain-containing protein 3, Hypoxia-inducible factor prolyl hydroxylase 3, HIF prolyl hydroxylase 3, HIF-PH3, PHD3, egl nine-like protein 3 isoform, EC 1.14.11.29, HPH-1, HPH-3.

EGLN3 is a member of the EGLN family of prolyl hydroxylases. Its main function is to catalyze the hydroxylation of a specific subunit of hypoxia-inducible factor-?, which then gets targeted for ubiquitination by a complex involving the VHL tumor suppressor. EGLN3 plays a crucial role in limiting the activation of HIFs, particularly HIF2A, during hypoxia. It is activated in cardiovascular and Hela cells under low oxygen conditions. Additionally, EGLN3 restricts glycolysis by hydroxylating PKM2 in hypoxia. In normal oxygen levels, it also controls the stability of ADRB2. Polynitrogen compounds inhibit EGLN3, possibly by binding to Fe2+ ions.

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  • Sku: PSB-pro-1143-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Egl Nine Homolog 3 (EGLN3) is a member of the Egl Nine Homolog (EGLN) family, which plays a significant role in cellular oxygen sensing and response. EGLN3, with a molecular weight of approximately X kDa, is involved in the regulation of hypoxia-inducible factor (HIF) stability, an essential transcription factor in the cellular adaptation to low oxygen levels. By hydroxylating specific proline residues on HIF, EGLN3 facilitates its recognition by the von Hippel-Lindau (VHL) tumor suppressor protein, leading to HIF degradation. This process is crucial for maintaining cellular homeostasis and has implications in various physiological and pathological conditions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The EGLN3 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 300mM NaCl, 5mM DTT, 2mM EDTA and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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EGLN3
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