EGFP

Alternative Names: GFP, Green fluorescent protein

Green Fluorescent Protein (GFP), produced by the jellyfish Aequorea Victoria, emits green light in the visible spectrum. This protein is widely used as a fluorescent tag in the production of chimeric proteins. GFP is expressed in various cell types and serves as a noninvasive marker in living cells and organisms. It has multiple applications, including tracking cell lineage, monitoring gene expression, and measuring protein-protein interactions. Enhanced GFP (eGFP) has specific mutations that enhance its fluorescence and folding efficiency at 37°C.

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  • Sku: PSB-pro-1606-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Enhanced Green Fluorescent Protein (EGFP) is a genetically engineered variant of Green Fluorescent Protein (GFP) that exhibits enhanced fluorescence properties. Belonging to the family of fluorescent proteins, EGFP is widely utilized as a molecular marker in various biological studies and imaging techniques. With a molecular weight of approximately 27 kilodaltons, EGFP offers a convenient and reliable means to visualize and track protein expression, localization, and dynamics within living cells. Its fluorescence emission at a wavelength of 509 nanometers makes EGFP a valuable tool in fluorescence microscopy and other applications requiring precise visualization of cellular components.

Current Lead Time

7-14 Days

Product Specifications

Species
Protein
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
26.9 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Lyophilized
Formulation The EGFP was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Storage Instruction

Lyophilized EGFP although stable at room temperature for 3 weeks, should be stored desiccated below -18<SUP>0</SUP>C. Upon reconstitution EGFP should be stored at 4<SUP>0</SUP>C between 2-7 days and for future use below -18<SUP>0</SUP>C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized EGFP in sterile distilled H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available

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EGFP

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