EEF2

Alternative Names: Elongation factor 2, Eukaryotic Translation Elongation Factor 2, EF-2, EEF-2, EF2, EEF2

Eukaryotic Translation Elongation Factor 2 (EEF2) is a crucial factor in protein synthesis and belongs to the GTP-binding translation elongation factor family. It plays a role in the GTP-dependent ribosomal translocation step during translation elongation. EEF2 also facilitates the coordinated movement of mRNA, two tRNA molecules, and conformational changes in the ribosome. The protein EEF2 is inactivated by phosphorylation of EF-2 kinase.

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  • Sku: PSB-pro-2076-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Eukaryotic Translation Elongation Factor 2 (eEF2) is a protein involved in the elongation phase of protein synthesis in eukaryotic cells. Belonging to the GTP-binding translation elongation factor family, eEF2 facilitates the translocation of the ribosome along the mRNA during protein synthesis. With a molecular weight of approximately 95 kDa, this protein plays a vital role in ensuring accurate and efficient translation of genetic information into functional proteins within the cell.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
34.3 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The EEF2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSDPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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EEF2

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