EDF1

Alternative Names: Multiprotein-bridging factor 1, EDF-1, MBF1.

EDF1 is a key regulator in the process of endothelial cell differentiation. It functions as a bridging protein, linking regulatory proteins to the basal transcriptional machinery, which ultimately affects the transcription of genes involved in endothelial differentiation. EDF1 interacts with calmodulin through its IQ domain and plays a role in controlling the activity of nitric oxide synthase by sequestering calmodulin in the cytoplasm. EDF1 is present in various tissues, including the liver, heart, adipose tissues, intestine, and pancreas.

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  • Sku: PSB-pro-494-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Endothelial Differentiation-Related Factor 1 (EDRF1) is a protein that plays a significant role in endothelial cell differentiation and vascular development. Belonging to the family of growth factors, EDRF1 is involved in regulating angiogenesis, the formation of new blood vessels. With a molecular weight of approximately X kDa, EDRF1 exerts its effects through binding to specific receptors on endothelial cells, activating intracellular signaling pathways that promote endothelial cell proliferation and migration. This recombinant protein holds potential in various research and therapeutic applications related to vascular biology and angiogenesis.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation 0.5 mg/ml solution containing 20mM Tris pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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EDF1

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