ECHS1

Alternative Names: SCEH, Enoyl-CoA hydratase 1

ECHS1 is a member of the hydratase/isomerase superfamily located in the mitochondrial matrix. It is primarily found in muscle, liver, and fibroblasts, with lower expression in the kidney and spleen. ECHS1 functions as a homohexamer involved in the second stage of the mitochondrial fatty acid β-oxidation pathway.

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  • Sku: PSB-enz-556-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial is an enzyme that plays a key role in fatty acid metabolism. Belonging to the enoyl-CoA hydratase family, this protein is responsible for catalyzing the hydration of enoyl-CoA intermediates during the beta-oxidation process within the mitochondria. With a molecular weight of approximately 33 kDa, Recombinant Human Enoyl CoA Hydratase is crucial for maintaining proper energy production and lipid homeostasis within the cell.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homohexamer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The ECHS1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT,0.1M NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ECHS1

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