DUSP3

Alternative Names: Dual specificity phosphatase 3, Dual specificity protein phosphatase VHR, serine/threonine specific protein phosphatase, Vaccinia virus phosphatase VH1-related, Vaccinia H1-related phosphatase, VHR

DUSP3 is a member of the dual specificity protein phosphatase subfamily that dephosphorylates both phosphoserine/threonine and phosphotyrosine residues, inactivating their target kinases. It negatively regulates mitogen-activated protein kinases involved in cellular proliferation and differentiation. DUSP3 is found in breast and ovarian tissues and shows strong preference for dephosphorylating and inactivating phosphotyrosines, particularly ERK1 and ERK2.

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  • Sku: PSB-enz-176-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human Dual Specificity Phosphatase 3 is a member of the protein tyrosine phosphatase family, specifically classified as a dual-specificity phosphatase. This enzyme plays a crucial role in the regulation of various cellular processes by dephosphorylating both tyrosine and serine/threonine residues on target proteins. With a molecular weight of approximately 42 kDa, Recombinant Human Dual Specificity Phosphatase 3 is commonly used in research settings to study signaling pathways and protein phosphorylation events.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DUSP3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DUSP3
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