• Home
  • DUSP18, Active

DUSP18, Active

Alternative Names: Dual specificity protein phosphatase 18, DUSP18, Low molecular weight dual specificity phosphatase 20, LMW-DSP20, LMWDSP20, VHP, DUSP26.

DUSP18, a member of the DSP family, primarily dephosphorylates tyrosine residues and has some activity on threonine residues. It also dephosphorylates pNPP and is inhibited by iodoarectic acid but activated by manganese ions. DUSP18 is expressed most prominently in the liver, brain, ovary, and testis.

  •
  • Sku: PSB-enz-1040-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Dual Specificity Phosphatase 18, Active is a member of the phosphatase enzyme family, specifically belonging to the dual specificity phosphatase subfamily. This enzyme plays a critical role in the regulation of cell signaling pathways by dephosphorylating both tyrosine and serine/threonine residues on target proteins. With a molecular weight of approximately 45 kDa, Recombinant Human Dual Specificity Phosphatase 18, Active is widely used in research laboratories for its ability to modulate cellular processes and study signal transduction mechanisms.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 300 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 23.6 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DUSP18 protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 0.1mM PMSF, 1mM DTT, 40% glycerol and 1mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

DUSP18, Active
SUBHEADING

Recently viewed products