DUSP10

Alternative Names: Dual specificity protein phosphatase 10, dual specificity phosphatase MKP-5, MKP-5, MAP kinase phosphatase 5, Mitogen-activated protein kinase phosphatase 5, serine/threonine specific protein phosphatase, EC 3.1.3.16, EC 3.1.3.48.

DUSP10, a member of the protein-tyrosine phosphatase family, inactivates target kinases by dephosphorylating phosphoserine/threonine and phosphotyrosine residues. It negatively regulates MAPK superfamily members associated with cellular proliferation and differentiation, interacting with MAPK14 and MAPK8. In mammalian cells, DUSP10 selectively blocks the enzymatic activation of MAP kinases, prioritizing p38 over JNK/SAPK and ERK.

  •
  • Sku: PSB-enz-238-5µg
  • Vendor: ProSpec-Tany
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UniProt Listing
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Product Details

Product Description

Recombinant Human Dual Specificity Phosphatase 10 (DUSP10) is a member of the dual-specificity phosphatase family, which plays a key role in the regulation of mitogen-activated protein kinase (MAPK) signaling pathways. DUSP10 is involved in the dephosphorylation of both tyrosine and threonine/serine residues on MAPKs, thereby modulating cellular responses to various stimuli. With a molecular weight of approximately 42 kDa, Recombinant Human DUSP10 is commonly used in research settings to study its impact on cell signaling and gene expression.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 40.4 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DUSP10 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DUSP10
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