DTD1

Alternative Names: bA379J5.3, bA555E18.1, C20orf88, DUEB, HARS2, pqn-68, D-tyrosyl-tRNA(Tyr) deacylase 1, DNA-unwinding element-binding protein B.

DTD1, a member of the DTD family, is an enzyme that hydrolyzes D-tyrosyl-tRNA(Tyr) to D-tyrosine and free tRNA(Tyr). It may act as a defense mechanism against the damaging effects of D-tyrosine and is involved in DNA replication by facilitating the loading of CDC45 onto pre-replication complexes. DTD1 localizes to active replication origins and is expressed in various tissues, with highest levels found in the testis, ovary, spleen, adult brain, and fetal brain. It has been suggested that DTD1 could be a risk factor for aspirin-intolerant asthma by promoting a pro-inflammatory phenotype through the hydrolysis of D-tryptophan and interaction with the tyrosyl-tRNA synthetase enzyme.

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  • Sku: PSB-enz-671-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human D-Tyrosyl-tRNA Deacylase 1 is an enzyme that belongs to the D-Tyr-tRNA deacylase family, which plays a role in the hydrolysis of mischarged D-aminoacyl-tRNAs in protein synthesis. This enzyme has a molecular weight of approximately 18 kDa and is involved in maintaining the fidelity of protein translation by removing mischarged tRNAs from the ribosome. Recombinant Human D-Tyrosyl-tRNA Deacylase 1 is a crucial component in ensuring accurate protein synthesis in cells.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 25.9 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation DTD1 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMKAVVQR VTRASVTVGG EQISAIGRGI CVLLGISLED TQKELEHMVR KILNLRVFED ESGKHWSKSVMDKQYEILCV SQFTLQCVLK GNKPDFHLAM PTEQAEGFYN SFLEQLRKTY RPELIKDGKF GAYMQVHIQN DGPVTIELES PAPGTATSDP KQLSKLEKQQ QRKEKTRAKG PSESSKERNT PRKEDRSASS GAEGDVSSER EP.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DTD1
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