DsbG

Alternative Names: b0604, JW0597, dsbG, ybdP, Thiol:disulfide interchange protein dsbG

Dsb proteins play a crucial role in the creation and adjustment of disulfide bonds while secreted and membrane proteins fold in bacteria. Specifically, DsbG acts as a disulfide bond isomerase and chaperone. In experiments, DsbG has been observed to interact with partially unfolded citrate synthase and prevent its clumping. DsbG shares genetic similarities with DsbC and forms a stable dimer in the periplasmic space. Furthermore, DsbG has a similar affinity for glutathione compared to DsbA and DsbC. It is worth noting that DsbG is expressed at approximately a quarter of the level of DsbC.

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  • Sku: PSB-hsp-024-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant E.Coli Thiol Disulfide Interchange Protein DsbG is a protein found in Escherichia coli bacteria that plays a significant role in the maintenance of proper protein folding and disulfide bond formation. Belonging to the family of thiol-disulfide oxidoreductases, DsbG assists in the reduction of incorrect disulfide bonds in proteins, ensuring their correct folding and functionality. With a molecular weight of approximately 20 kDa, this protein is involved in the intricate process of disulfide bond rearrangement, contributing to the overall stability and functionality of proteins in E. coli.

Current Lead Time

7-14 Days

Product Specifications

Species
 E. Coli
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DsbG protein solution contains 20mM Tris-HCl, pH-8, 2mM EDTA and 10% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DsbG
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