DsbA

Alternative Names: Thiol:disulfide interchange protein dsbA, DsbA

DsbA plays a crucial role in forming disulfide bonds in exported proteins in vivo. It is commonly used as a standard in immunoblotting due to its ability to catalyze the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. DsbA is known as the most potent oxidant in the thioredoxin superfamily and is necessary for efficient disulfide bond formation in the periplasm of E. coli.

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  • Sku: PSB-enz-276-10µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Disulfide Oxidoreductase is an enzyme that plays a key role in catalyzing the formation and isomerization of disulfide bonds in proteins. Belonging to the thioredoxin family, this enzyme is essential for maintaining the correct protein structure and function. With a molecular weight of approximately 35 kDa, Recombinant Disulfide Oxidoreductase is involved in redox reactions within the cell, contributing to the stability and integrity of proteins.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Lyophilized
Formulation The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys-Ala-Trp.

Storage Instruction

Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized DsbA in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available

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DsbA

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