DPH2

Alternative Names: DPH2, DPH2 Homolog, DPH2-Like 2, DPH2L2, Diphtheria Toxin Resistance Protein 2, Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2, Diphthamide Biosynthesis Protein 2, Diphthamide Biosynthesis Protein 2 Homolog-Like 2, Diphthamide Biosynthesis-Like Protein 2, EC 2.5.1.108, S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 2, 2-(3-Amino-3-Carboxypropyl)Histidine Synthase Subunit 2.

Diphthamide biosynthesis protein 2 (DPH2) is a homodimer that binds a [4Fe-4S] cluster. It is targeted by ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). DPH2 was found to complement a diphthamide mutant strain, contributing to diphthamide biosynthesis. In the absence of DPH2, NF-kB and death receptor pathways are activated, making MCF7 cells highly sensitive to tumor necrosis factor.

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  • Sku: PSB-pro-2421-1µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Diphthamide Biosynthesis 2 (RHDBS2) is a protein involved in the biosynthesis of diphthamide, a unique post-translational modification found in eukaryotic translation elongation factor 2 (eEF2). RHDBS2 belongs to the family of proteins known as diphthamide biosynthesis proteins, which play a crucial role in the addition of diphthamide to eEF2. With a molecular weight of approximately [insert weight], RHDBS2 acts as an integral component in the enzymatic pathway responsible for the formation of diphthamide, a process critical for proper protein synthesis and cellular function.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 85.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
53.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation DPH2 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DPH2

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