DNMT3L

Alternative Names: DNA (Cytosine-5-)-Methyltransferase 3-Like, DNA (Cytosine-5)-Methyltransferase 3-Like, Human Cytosine-5-Methyltransferase 3-Like Protein 11, Cytosine-5-Methyltransferase 3-Like Protein, DNMT3L

DNMT3L is a nuclear protein with similarity to DNA methyltransferases. It does not possess the necessary amino acid residues for methyltransferase activity. DNMT3L is believed to stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be necessary for the formation of maternal genomic imprints. Additionally, DNMT3L interacts with histone deacetylase 1 to mediate transcriptional repression. As a catalytically inactive regulatory factor, DNMT3L is essential for the function of DNA methyltransferases 3A and 3B. By binding to the catalytic domain of these methyltransferases, DNMT3L activates them. It also facilitates the binding of DNA and AdoMet to the methyltransferases before dissociating from the complex.

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  • Sku: PSB-enz-787-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human DNA Cytosine-5--Methyltransferase 3-Like (DNMT3L) is a member of the DNA methyltransferase family, which plays a key role in the regulation of gene expression through DNA methylation. This protein is involved in the establishment of genomic methylation patterns during early embryonic development. DNMT3L has a molecular weight of approximately 45 kDa and is essential for proper epigenetic programming in mammalian cells. Its function is crucial for maintaining genomic stability and proper gene regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
46.2 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DNMT3L solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DNMT3L

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