DNase

Alternative Names: Deoxyribonuclease I, DNase I, DNASE1, DRNI, DNL1, EC 3.1.21.1, FLJ38093

RhDNase is a recombinant human enzyme that selectively cleaves DNA by splitting phosphodiester linkages in polynucleotides. It primarily targets single stranded DNA (ssDNA), double stranded DNA (ddDNA), and chromatin, and is activated by bivalent metals like Mg+2 and Ca+2. Commonly used in biochemical methods, RhDNase is used to digest DNA and recover RNA without impacting structural proteins or enzymes. In tissue culture, RhDNase is used to reduce viscosity by digesting DNA from damaged cells and remove membrane-bound DNA fragments.

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  • Sku: PSB-enz-319-500IU
  • Vendor: ProSpec-Tany
$65.00 USD
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Title

Product Details

Product Description

Recombinant Human Deoxyribonuclease I is a type of enzyme that belongs to the DNase family. It is commonly used in molecular biology and biochemistry research to cleave DNA molecules. With a molecular weight of approximately 33 kDa, Recombinant Human Deoxyribonuclease I is an important tool in the study of DNA structure and function. Its ability to specifically target and degrade DNA makes it a valuable asset in various laboratory experiments and techniques.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System CHO
Purity
Activity
Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.

*For Research Use Only

Notes

Specific Activity: 1000IU/1mg

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DNase

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