Dnak LCS

Alternative Names: HSP-70, HSP70, Heat shock protein 70, Heat shock 70 kDa protein, DnaK, Chaperone protein dnaK, groP, grpF, seg, b0014, JW0013.

DnaK, originally discovered for its role in DNA replication by bacteriophage l in E. coli, is a bacterial hsp70 chaperone. It aids in folding and assembling newly synthesized polypeptide chains and prevents stress-denatured proteins from aggregating. The substrate binding domain of DnaK (residues 508-638) consists of alpha helices and acts as a lid over the substrate binding cleft. A region of DnaK containing amino acids 508-638, with an additional N-terminal methionine, was purified using standard column chromatography techniques.

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  • Sku: PSB-hsp-011-20µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

The Recombinant E.Coli Dnak Lid Covering Substrate is a protein molecule that belongs to the family of heat shock proteins (HSPs). With a weight of approximately X kilodaltons, this substrate plays a significant role in the folding and unfolding of proteins within the cell. It acts as a protective cover for the DnaK chaperone, preventing the binding of unfolded proteins and facilitating their proper refolding. This Recombinant E.Coli Dnak Lid Covering Substrate is a crucial component in maintaining cellular protein homeostasis and ensuring proper protein function.

Current Lead Time

7-14 Days

Product Specifications

Species
 E. Coli
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIESALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADASANNAKDDDVVDAEFEEVKDKK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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Dnak LCS
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