DLAT

Alternative Names: Pyruvate dehydrogenase complex component E2, Dihydrolipoamide S-Acetyltransferase, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, M2 antigen complex 70 kDa subunit, DLAT, EC 2.3.1.12, EC 2.3.1.

The DLAT gene encodes the enzyme E2 in the pyruvate dehydrogenase complex (PDC) found in the inner mitochondrial membrane. This enzyme converts pyruvate to acetyl coenzyme A. Dihydrolipoamide acetyltransferase, the protein product of DLAT, transfers acetyl groups from pyruvate to coenzyme A. Antimitochondrial antibodies target this protein in patients with primary biliary cirrhosis (PBC), where activated T lymphocytes attack bile duct epithelial cells due to abnormal distribution and overexpression of dihydrolipoamide acetyltransferase. PBC leads to liver cirrhosis and failure. Mutations in DLAT can also cause pyruvate dehydrogenase E2 deficiency, resulting in primary lactic acidosis in infants and young children.

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  • Sku: PSB-enz-082-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Dihydrolipoamide S-Acetyltransferase is a protein belonging to the transferase family, specifically the acyltransferase subfamily. It plays a key role in the catalysis of the transfer of acetyl groups from a thiamine pyrophosphate-bound substrate to CoA. With a molecular weight of approximately 78 kDa, this enzyme is crucial for the proper functioning of the citric acid cycle and fatty acid metabolism.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Insect cells
Purity 75% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation DLAT (0.91mg/ml)is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DLAT
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