DHPS

Alternative Names: EC 2.5.1.46, Deoxyhypusine synthase, DHS, DHPS, DS, MIG13.

DHPS is a crucial enzyme in the initial stage of hypusine biosynthesis. It transfers the butylamine moiety from spermidine to a specific lysine residue on the EIF5A precursor protein, forming the deoxyhypusine residue.

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  • Sku: PSB-enz-498-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Deoxyhypusine Synthase is an enzyme that plays a key role in the post-translational modification of eukaryotic translation initiation factor 5A (eIF5A). Belonging to the transferase family, this enzyme catalyzes the formation of hypusine, a unique amino acid found only in eIF5A. With a molecular weight of approximately 24 kDa, Recombinant Human Deoxyhypusine Synthase is involved in regulating protein synthesis and cell proliferation. Its precise function in cellular processes makes it a valuable target for research in the field of molecular biology.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DHPS solution contains 20mM Tris-HCl pH-8, 0.1M NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNED.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DHPS

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