DFFA

Alternative Names: DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45.

DFF is a protein consisting of two subunits, DFFA and DFFB, with molecular weights of 45kDa and 40kDa, respectively. DFFA is acted upon by caspase-3, leading to DNA fragmentation during apoptosis. Activation of DFF occurs after caspase-3 cleaves DFFA. Once cleaved, the fragments of DFFA dissociate from DFFB, the active component of DFF. This dissociation triggers DNA fragmentation and chromatin condensation during apoptosis. Apoptosis involves cell and nuclear shrinkage, fragmentation, and degradation of chromosomal DNA into nucleosomal units. In ovarian endometriosis, a lower level of DFFA may contribute to an apoptosis-resistant mechanism, thereby promoting disease progression. Rare allelic variants of DFFA have also been observed in neuroblastoma tumors located on chromosome 1.

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  • Sku: PSB-pro-718-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human DNA Fragmentation Factor Subunit Alpha, also known as DFFA, is a protein that plays a crucial role in DNA fragmentation during apoptosis, the programmed cell death process. Belonging to the DFF family, this protein acts as a catalyst, aiding in the cleavage of DNA into smaller fragments. With a molecular weight of approximately X kDa, DFFA is involved in the disassembly of chromatin structure and is essential for the proper execution of apoptosis. Understanding the functions and characteristics of this protein is of significant interest in various scientific studies and biomedical research.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
38.7 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DFFA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DFFA

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