DESI1

Alternative Names: Desumoylating Isopeptidase 1, Desumoylating Isopeptidase 2, Family With Sequence Similarity 152 Member B, FAM152B, PPPDE Peptidase Domain-Containing Protein 2, PPPDE2, DeSI-1, DESI2, D15Wsu75e, DJ347H13.4, EC 3.4.-.-.

DESI1, a member of the DeSI family, possesses a single PPPDE peptidase domain and functions as a protease that deconjugates SUMO1, SUMO2, and SUMO3 from various substrate proteins. It exhibits isopeptidase activity but lacks SUMO-processing activity. DESI1 is known to desumoylate the protein ZBTB46.

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  • Sku: PSB-enz-734-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Desumoylating Isopeptidase 1, also known as SENP1, is a member of the SENP family of proteases. It is responsible for the removal of small ubiquitin-like modifier (SUMO) proteins from target proteins, thereby regulating various cellular processes. With a molecular weight of approximately 74 kDa, SENP1 plays a critical role in maintaining protein homeostasis and cellular function. Its precise enzymatic activity and specificity make it a valuable target for research in the field of post-translational modifications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
20.7 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DESI1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEPPNLY PVKLYVYDLS KGLARRLSPI MLGKQLEGIW HTSIVVHKDE FFFGSGGISS CPPGGTLLGP PDSVVDVGST EVTEEIFLEY LSSLGESLFR GEAYNLFEHN CNTFSNEVAQ FLTGRKIPSY ITDLPSEVLS TPFGQALRPL LDSIQIQPPG GSSVGRPNGQ S

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DESI1

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