DDT

Alternative Names: Not Available

DDT is an enzyme that converts D-dopachrome to 5,6-dihydroxyindole. It belongs to the lyase family, specifically carboxy-lyases, which break carbon-carbon bonds. DDT has a 33% identical amino acid sequence with MIF and similar tautomerase activity. It acts as a proinflammatory cytokine.

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  • Sku: PSB-enz-527-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human D-Dopachrome Tautomerase is a protein belonging to the tautomerase family. It plays a role in the melanin biosynthesis pathway by catalyzing the conversion of D-dopachrome to 5,6-dihydroxyindole-2-carboxylic acid. This protein has a molecular weight of approximately 12 kDa. Recombinant Human D-Dopachrome Tautomerase is commonly used in research studies related to melanogenesis and skin pigmentation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation DDT Human solution containing 20mM Tris-HCl pH-8, & 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DDT

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