DDIT4

Alternative Names: DNA-damage-inducible transcript 4, RTP801, REDD-1, Dig2, FLJ20500, Protein regulated in development and DNA damage response 1, HIF-1 responsive protein RTP801, RP11-442H21.1.

In the regulation of reactive oxygen species, DDIT4 plays a crucial role. It is upregulated at the transcriptional level in response to stress caused by DNA damage and glucocorticoid treatment. As a negative regulator of the serine/threonine kinase mTOR, also known as the mammalian target of Rapamycin, DDIT4 serves a vital function in connecting external and internal signals for the regulation of mTOR.

  •
  • Sku: PSB-pro-094-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human DNA Damage Inducible Transcript 4 (DDIT4) is a protein belonging to the family of DNA damage-inducible transcripts. With a molecular weight of approximately 25 kilodaltons, DDIT4 plays a regulatory role in cellular stress response pathways. It is involved in the inhibition of the mammalian target of rapamycin complex 1 (mTORC1), a key regulator of cell growth and metabolism. DDIT4's interaction with mTORC1 helps modulate cellular processes such as autophagy and protein synthesis, making it a significant player in maintaining cellular homeostasis under stress conditions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 27.5 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DDIT4 protein solution (0.25mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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DDIT4
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