DDIT3

Alternative Names: DNA damage-inducible transcript 3, DDIT-3, Growth arrest and DNA-damage-inducible protein GADD153, C/EBP-homologous protein, CHOP, GADD153, DDIT3, CEBPZ, CHOP10, MGC4154.

DDIT3, also known as GADD153, is a bZIP transcription factor that belongs to the C/EBP family. It forms heterodimers with C/EBP and lap proteins, reducing their DNA-binding activity. This prevents the formation of DNA-binding complexes. DDIT3 expression is increased in response to various stresses, such as starvation, ER stress, osmotic stress, and hypoxia. Its role in ER stress-mediated apoptosis has been well established, and it has also been implicated in several diseases, including diabetes, brain ischemia, and neurodegenerative disease. DDIT3 is involved in asoprisnil-induced apoptosis and plays a role in hypoglycemia-induced necrotic cell death. Additionally, it has been shown to contribute to the progression of melanoma and is induced by HRG stimulation in mammary epithelial cells.

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  • Sku: PSB-pro-638-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human DNA Damage Inducible Transcript 3 (DDIT3), also known as CHOP (C/EBP homologous protein), is a member of the C/EBP family of transcription factors. With a molecular weight of approximately 29 kilodaltons, DDIT3 plays a significant role in cellular stress responses, particularly in regulating apoptosis and the unfolded protein response. This protein is involved in various physiological and pathological processes, including DNA damage, endoplasmic reticulum stress, and adipogenesis. Understanding the functions and mechanisms of DDIT3 can provide valuable insights into the cellular stress response and its implications in human health and disease.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Heterodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DDIT3 protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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DDIT3
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