DCTN2 (1-403)

Alternative Names: Dynactin 2 (P50), Dynactin Complex 50 KDa Subunit, P50 Dynamitin, DCTN50, 50 KDa Dynein-Associated Polypeptide, 50 KD Dynein-Associated Polypeptide, Dynactin Complex 50 KD Subunit, Epididymis Secretory Protein Li 77, DYNAMITIN, HEL-S-77, DCTN-50, RBP50.

DCTN2 is a component of the 10-11 subunit dynactin complex, which binds to both microtubules and cytoplasmic dynein. This complex is involved in various cellular processes, such as ER-to-Golgi transport, the movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. DCTN2 is found in 4-5 copies per dynactin molecule and is composed of 3 short alpha-helical coiled-coil domains that enable interaction with other dynactin subunits. Specifically, it directly interacts with the largest subunit (p150) of dynactin, facilitating its attachment. DCTN2 also regulates cytoplasmic dynein binding to an organelle and contributes to prometaphase chromosome alignment and spindle organization during mitosis. Additionally, DCTN2 is responsible for anchoring microtubules to centrosomes and plays a role in synapse formation during brain development.

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  • Sku: PSB-pro-2303-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Dynactin 2 (1-403 a.a.) is a protein belonging to the dynactin family, which plays a significant role in intracellular transport processes. With a molecular weight of approximately X kDa, this protein is involved in the regulation of cytoplasmic dynein, a motor protein responsible for the movement of various cellular cargoes along microtubules. Recombinant Human Dynactin 2 (1-403 a.a.) is a valuable tool for studying the molecular mechanisms underlying intracellular transport and can be utilized in various research applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 46.9 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation DCTN2 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEL EELTSTSVEH IIVNPNAAYD KFKDKRVGTK GLDFSDRIGK TKRTGYESGE YEMLGEGLGV KETPQQKYQR LLHEVQELTT EVEKIKTTVK ESATEEKLTP VLLAKQLAAL KQQLVASHLE KLLGPDAAIN LTDPDGALAK RLLLQLEATK NSKGGSGGKT TGTPPDSSLV TYELHSRPEQ DKFSQAAKVA ELEKRLTELE TAVRCDQDAQ NPLSAGLQGA CLMETVELLQ AKVSALDLAV LDQVEARLQS VLGKVNEIAK HKASVEDADT QSKVHQLYET IQRWSPIAST LPELVQRLVT IKQLHEQAMQ FGQLLTHLDT TQQMIANSLK DNTTLLTQVQ TTMRENLATV EGNFASIDER MKKLGK

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DCTN2 (1-403)
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