DCTN2 (1-401)

Alternative Names: DCTN2, DCTN50, Dynactin Subunit 2, Dynactin Complex 50 KDa Subunit, Dynactin Complex 50 KD Subunit, 50 KDa Dynein-Associated Polypeptide, 50 KD Dynein-Associated Polypeptide, P50 Dynamitin, DYNAMITIN, HEL-S-77, RBP50, Epididymis Secretory Protein Li 77, DCTN-50.

DCTN2 is a component of dynactin, a macromolecular complex including 10-11 subunits of varying sizes (22-150 kDa). Dynactin binds to microtubules and cytoplasmic dynein and has various cellular functions such as transport from the ER to the Golgi apparatus, movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. DCTN2 is found in 4-5 copies per dynactin molecule and consists of 3 alpha-helical coiled-coil domains that facilitate its interaction with other dynactin subunits. DCTN2 directly interacts with the largest dynactin subunit (p150) and stabilizes its position. DCTN2 regulates the binding of cytoplasmic dynein to an organelle and contributes to chromosome alignment and spindle organization during mitosis. Additionally, DCTN2 plays a part in anchoring microtubules to centrosomes and is involved in synapse formation during brain development.

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  • Sku: PSB-pro-1776-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Dynactin 2 (1-401 a.a.) is a protein that belongs to the dynactin family. With a weight of approximately 45 kDa, this protein plays a significant role in intracellular transport processes. It is involved in the regulation of cytoplasmic dynein, a motor protein responsible for the movement of various cellular cargoes along microtubules. Recombinant Human Dynactin 2 (1-401 a.a.) is widely studied for its involvement in cellular trafficking and its potential implications in various pathological conditions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 46.6 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation DCTN2 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0), 20% glycerol, 0.15M NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEE LTSTSVEHII VNPNAAYDKF KDKRVGTKGL DFSDRIGKTK RTGYESGEYE MLGEGLGVKE TPQQKYQRLL HEVQELTTEV EKIKTTVKES ATEEKLTPVL LAKQLAALKQ QLVASHLEKL LGPDAAINLT DPDGALAKRL LLQLEATKNS KGGSGGKTTG TPPDSSLVTY ELHSRPEQDK FSQAAKVAEL EKRLTELETA VRCDQDAQNP LSAGLQGACL METVELLQAK VSALDLAVLD QVEARLQSVL GKVNEIAKHK ASVEDADTQS KVHQLYETIQ RWSPIASTLP ELVQRLVTIK QLHEQAMQFG QLLTHLDTTQ QMIANSLKDN TTLLTQVQTT MRENLATVEG NFASIDERMK KLGK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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DCTN2 (1-401)
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