DCK

Alternative Names: DCK, Deoxycytidine kinase, MGC117410, MGC138632.

DCK plays a crucial role in salvaging deoxyribonucleosides and activating important nucleoside analogues. It catalyzes the 5`-phosphorylation of purine and pyrimidine deoxynucleosides using ATP or UTP as phosphate donors. Deficiency in DCK enzyme activity can lead to resistance to antiviral and anticancer drugs, while increased activity can enhance the activation of these drugs into cytotoxic compounds.

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  • Sku: PSB-pka-313-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Deoxycytidine Kinase is an enzyme that plays a key role in the activation of nucleoside analog prodrugs used in cancer chemotherapy. Belonging to the nucleoside phosphorylase family, this enzyme is responsible for the phosphorylation of deoxycytidine and other analogs, converting them into active cytotoxic compounds. With a molecular weight of approximately 30 kDa, Recombinant Human Deoxycytidine Kinase is a crucial component in the metabolism of nucleoside analogs in cancer cells.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DCK solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 7.5), 1mM DTT, 0.1mM PMSF, 2mM EDTA and 10% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DCK

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