DCAF7

Alternative Names: DDB1- and CUL4-associated factor 7, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog, DCAF7, HAN11, WDR68, AN11, SWAN-1.

DCAF7, a protein associated with DDB1 and CUL4, plays a key role in craniofacial development. It is essential for the formation of the upper jaw equivalent, known as the palatoquadrate, and acts upstream of the EDN1 pathway. The activity required for the function of the EDN1 pathway differs between the first and second arches. DCAF7 interacts with DIAPH1, influencing the transcriptional activity of GLI1. Additionally, DCAF7 may be involved in both normal and diseased skin development.

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  • Sku: PSB-pro-909-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human DDB1 and CUL4 Associated Factor 7 (DCAF7) is a protein that belongs to the WD repeat-containing protein family. With a molecular weight of approximately X kDa, DCAF7 plays a significant role in various cellular processes. It acts as a substrate receptor for the CUL4-DDB1 E3 ubiquitin ligase complex, facilitating the targeted degradation of specific proteins. DCAF7's involvement in protein degradation pathways highlights its importance in maintaining cellular homeostasis. By providing a platform for protein-protein interactions, DCAF7 contributes to the regulation of diverse biological functions.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
33.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation DCAF7 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.4M urea and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DCAF7

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