DBR1

Alternative Names: Debranching RNA Lariats 1, RNA Lariat Debranching Enzyme, Debranching Enzyme Homolog 1, Lariat Debranching Enzyme, EC 3.1.-.-.

DBR1 is an enzyme that breaks down 2'-5' branched phosphodiester bonds in RNA lariats. This enzyme specifically acts on the bonds located at the branch point of removed lariat intron RNA, converting them into linear molecules that are subsequently degraded. DBR1 also plays a role in retroviral replication.

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  • Sku: PSB-pro-1708-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Debranching RNA Lariats 1, a member of the debranching enzyme family, is a bioengineered molecule designed for specific RNA processing applications. With a precise molecular weight, this recombinant protein exhibits enzymatic activity that aids in the removal of branched RNA structures, thereby facilitating the accurate processing of RNA molecules. Its application in various research areas makes it a valuable tool for scientists and researchers studying RNA biology and related fields.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
38.0 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The DBR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DBR1

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